Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry.

نویسندگان

  • Narasimha V Hegde
  • Bhushan M Jayarao
  • Chitrita DebRoy
چکیده

Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10(3) target cells in a bacterial mixture of 10(5) CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 50 6  شماره 

صفحات  -

تاریخ انتشار 2012